Life Sci. 2015 May 1;128:39-46. doi: 10.1016/j.lfs.2015.02.018. Epub 2015 Mar 4.
Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice.
Jung MK1, Ha S1, Huh SY2, Park SB3, Kim S3, Yang Y4, Kim D5, Hur DY5, Jeong H6, Bang SI7, Park H8, Cho D9.
1Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-ku, Seoul 140-742, Republic of Korea.
2Department of Biological Sciences, University of Connecticut, CT 06269-3042, USA.
3Biotech. Team, Cent'l Res. Inst., Ilyang Pharm., Co., Ltd., 359, Giheung-gu, Yongin-si, Gyeonggi-do, 449-726, Republic of Korea.
4Department of Plastic Surgery, College of Medicine, Sungkyunkwan University, Seoul 110-745, Republic of Korea.
5Department of Anatomy, Inje University College of Medicine, Pusan 614-735, Republic of Korea.
6Department of Chemistry, Sookmyung Women's University, Cheongpa-ro 47-gil 100, Yongsan-Ku, Seoul 140-742, Republic of Korea.
7Department of Plastic Surgery, College of Medicine, Sungkyunkwan University, Seoul 110-745, Republic of Korea. Electronic address: firstname.lastname@example.org.
8Department of Dermatology, St. Mary's Hospital, The Catholic University, Seoul 137-701, Republic of Korea. Electronic address: email@example.com.
9Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-ku, Seoul 140-742, Republic of Korea. Electronic address: firstname.lastname@example.org.
Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability.
To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting.
The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduced by an ERK1/2 inhibitor and the level of ERK1/2 phosphorylation was increased by treatment with Vd3.
This has been the first report on CM from Vd3 pre-activated preadipocyte displaying stimulatory effects on hair growth via the enhancement of angiogenesis in a hairless-induced C57BL/6 mice.
Copyright © 2015 Elsevier Inc. All rights reserved.
Angiogenesis; Extracellular signal-regulated kinase 1/2; Hair growth; Preadipocyte; Vitamin D3